Measuring the dependence of the yeast heat shock response on intracellular pH during stress

S. cerevisiae cells acidify when they experience stressful temperatures. To determine whether this heat-shock associated acidification is important for the cellular response, we manipulated intracellular pH, heat shocked cells, and sequenced the transcriptome.酿酒酵母（S. cerevisiae）细胞在遭遇胁迫性温度时会发生酸化。为明确该热激相关酸化过程对细胞应激响应的重要性，我们对细胞内pH值进行了调控、对细胞实施热激处理，并对其转录组进行了测序。