Effect of electroacupuncture on the skeletal muscle atrophy induced by hindlimb suspension in mice

The effect of electroacupuncture on the transcriptional profile of the skeletal muscle atrophy induced by hindlimb suspension were examined. We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated and down-regulated genes during this process. Total RNA samples were isolated from soleus muscle obtained from mice. The RNA was converted to biotin-labeled cRNA, which was hybridized to Affymetrix 1.0 ST array. The hybridization experiments were performed in duplicate.