Lesion-remote astrocytes govern microglia-mediated white matter repair III

Single Nuclei RNA seq was used to examine gene expression of all spinal cord cell types from rostral and caudal spared regions of the injured spinal cord in a mouse model of incomplete spinal cord injury (iSCI, lateral hemisection) at multiple timepoints after injury (3, 7, 28 dpi). A focus of this study was to characterize spatiotemportal lesion-remote astrocyte gene expression via integration with paired spatial transcriptomics data. Overall design: Cohorts of WT mice were perfused at 3, 7, or 28 days post injury, spinal cords dissected out and 3mm chunks of spared spinal cord tissue rostral and cuadal to the injury site were dissected out, flash frozen and processed for nuclei isolation. Equivalent tissue was taken from uninjured healthy control mice.