Metadata record for the article: Signaling of MK2 Sustains Robust AP1 Activity for Triple Negative Breast Cancer Tumorigenesis through Direct Phosphorylation of JAB1

Summary This metadata record provides details of the data supporting the claims of the related article: “Signaling of MK2 Sustains Robust AP1 Activity for Triple Negative Breast Cancer Tumorigenesis through Direct Phosphorylation of JAB1”. The related study showed that p38MAPK signalling pathway regulation of activator protein 1 (AP1) activity involves both MAPKAPK2 (MK2) and JAB1, a known JUN binding protein. Type of data: signalling pathway activity Subject of data: antibodies; Eukaryotic cell lines (ATCC); Mus musculus (Foxn1nu, female, Catalog# 007850, The Jackson laboratory) Data access The following files underlying the figures in the related manuscript are openly available with this data record: - Fig.1 data.xlsx (Fig.1a,1b of the related article) - Fig.2 data.xlsx (Fig.2c,2d and 2e) - Fig.3 data.xlsx (Fig.3e and 3f) - Fig.4 data.xlsx (Fig.4f,4g and 4f) - Fig.5 data.xlsx (Fig.5b,5c and 5d) - Fig.6 data.xlsx - Fig.7 data.xlsx (Fig. 7e and 7f) - Fig.8 data.xlsx (Fig.8a and 8c) All other data supporting the related study can be found in the supplementary information file of the related article, and the corresponding author can make any materials available upon request. Un-cropped gels and western blots for Fig. to Fig.5 were included in Supplementary Materials (Fig.S11).” JAB1 expression in different breast cancer subtypes were downloaded from https://tcga.xenahubs.net/download/TCGA.BRCA.sampleMap/HiSeqV2.gz, and https://tcga.xenahubs.net/download/TCGA.BRCA.sampleMap/BRCA_clinicalMatrix.gz. For analysis of p38MAPK activity in breast cancer, Reverse Phase Protein Array (RPPA) z score and corresponding clinical data from TCGA Breast Cancer Invasive Carcinoma, PanCancer Atlas were first downloaded through cBioportal (https://www.cbioportal.org/). Corresponding author(s) for this study Shuang Huang, Department of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, FL 32610. E-mail: [e-mail] Study approval University of Florida Institutional Animal Care and Use Committee.