The HIFI reads and assembled contigs used to construct the mitochondrial genome assembly of Glyphodes pyloalis

Glyphodes pyloalis (Lepidoptera; Crambidae; Spilomelinae), known as the mulberry pyralid, is a notorious insect pest belonging to the Lepidopteran that threatens mulberry cultivation across China. The mitochondrial genome is an invaluable genetic resource owing to its maternal inheritance, rapid evolution, and lack of recombination. A previous study documented the G. pyloalis mitochondrial genome (mtDNA) as NC_025933 using short-read next-generation sequences that, while pioneering, leaves room for improvement with modern long-read sequencing. PacBio’s high-fidelity circular consensus sequencing (HIFI CCS) generates extra-long sequences sufficient to produce complete mtDNA assemblies with base pair accuracy.