Supplemental_tables.pdf

Supplemental tables. Table S1 provides DNA diagnostics of patients with MC4R variants. Table S2 provides the cloning and site-directed mutagenesis primers used to create the MC4R primers in the respective expression plasmids. Table S3 provides additional characteristics of the 11 pediatric patients with MC4R variants. Table S4 provides additional characteristics of the 6 adult patients with MC4R variants. Table S5 provides EC50 and Emax obtained from α-MSH and setmelanotide induced cAMP response. Table S6 provides EC50 and Emax obtained from α-MSH and setmelanotide induced β-arrestin-2 recruitment. Table S7 provides Emax obtained from the quantification of cell surface expression. Table S8 provides Emax obtained from the quantification of total expression. Table S9 provides EC50 and Emax obtained from α-MSH and setmelanotide induced ERK activation.