Partial urethra obstruction in rat

Urethra was partially ligated and the urinary bladder was removed 10 days or 6 weeks after obstruction. Sham operated rats were used as controls. An addtitonal group of rats were repoerated 6 weeks after surgery and the obstruction was removed. These rats were then sacrificed 10 days after deobstruction. The bladder (including the urothelium) was frozen and used for RNA extraction.对大鼠尿道实施部分结扎，于尿路梗阻造模后10天或6周摘除膀胱。以假手术组大鼠作为实验对照。另设一组大鼠于术后6周行二次手术，解除尿路梗阻，并于解除梗阻后10天处死该组大鼠。采集的膀胱组织（含尿路上皮（urothelium））经速冻保存后用于RNA提取。