Agropyron mongolicum cultivar:Agropyron mongolicum keng Genome sequencing and assembly

The seeds of Agropyron mongolicum Keng were collected from Salaqi Agropyron mongolicum Keng experimental base Inner Mongolia Agricultural University Inner Mongolia Autonomous Region China Select full seeds remove lemma disinfect them with sodium hypochlorite solution for 15 minutes then wash them with sterilized distilled water for 5 times put them into germination box and cultivate them in indoor incubator set the temperature at 24 degrees centigrade photoperiod for 16 hours supplement evaporation water every day until the seedlings grow to the stage of three leaves one heart The leaves of Agropyron mongolicum Keng treated with drought for 12 h 24 h 48 h 3 D 5 d 7 d and rewatered for 24 h were sampled The leaves without drought treatment were set as control and stored in liquid nitrogen in pre cooled cryopreservation tube it was quickly put into the pre freezing tube and stored in liquid nitrogen