Dorsal hippocampal transcriptome analysis after Auditory Fear Conditioning and pharmacological GR modulation. Dorsal hippocampal transcriptome analysis after Auditory Fear Conditioning and pharmacological GR modulation

Aim: We investigated the role of GR in Auditory Fear Conditioning memory consolidation by application of a pharmacological filter using selective glucocorticoid receptor modulators. Methods: Adult male mice were exposed to Auditory Fear Conditioning training and subsequently injected with 3.0 mg/kg corticosterone, 20 mg/kg CORT108297, 80 mg/kg CORT118335, 40 mg/kg RU486 or vehicle. Dorsal hippocampi were dissected 3 hours after injection and snap-frozen. Total RNA was isolated and send for 100bp paired-end sequencing by BGI. Overall design: Hippocampal mRNA profiles of male mice after Auditory Fear Conditioning combined with multiple GR-ligands.