Julien Mouysset, Alexandra Deichsel, Sandra Moser, Carsten Hoege, Anthony A. Hyman, Anton Gartner, Thorsten Hoppe (2011) CIL:25632, Caenorhabditis elegans, early embryonic cell. CIL. Dataset

This movie illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of npl-4 through RNAi. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Time-lapse differential interference contrast (DIC) microscopy identified that downregulation of cdc-48, ufd-1, and npl-4 increases the cell division delay of P1 in comparison to AB, leading to a prolonged three-cell stage. The movie corresponds to experiments shown in Fig. 1B and Movie 5 of Mouysset et al. Movie is CIL 25632 and original data file is CIL 35146.

本电影展示了通过RNA干扰去除npl-4的C. elegans胚胎在四细胞阶段的早期分裂过程。C. elegans受精卵P0的第一次胚胎分裂是不对称的，生成了一个前端的AB细胞以及一个较小的后端P1细胞。这些细胞具有不同的发育命运和分裂时间，AB细胞分裂时间大约在P1细胞之前2分钟。时间分辨差分干涉对比（DIC）显微镜检测到cdc-48、ufd-1和npl-4的下调增加了P1细胞的分裂延迟，与AB细胞相比，导致三细胞阶段延长。该电影对应于Mouysset等人图1B和电影5中展示的实验。电影编号为CIL 25632，原始数据文件编号为CIL 35146。