Nanoliter-scale next-generation sequencing library mediated high-throughput 16s RRNA microbial community profiling: Supplementary Tables

<b>Supplementary Table 1.
</b> Nanoliter-scale
next-generation sequencing library mediated high-throughput 16s RRNA
microbial community profiling.<br>

Primer sequences. The
inner primer pairs were used to amplify the V3/V4 region of the 16S rRNA
gene. The outer primers with spacer sequences and SP sequences at the
5´region of primer were used to construct SNAP-TE libraries compatible for
Illumina MiSeq/HiSeq platform.

<b>Supplementary Table 2.
</b> Nanoliter-scale
next-generation sequencing library mediated high-throughput 16s RRNA
microbial community profiling.

Index sequences used
for next-generation sequencing library construction.

<b>Supplementary Table 3.
</b> Nanoliter-scale
next-generation sequencing library mediated high-throughput 16s RRNA
microbial community profiling.

A total of 14
flexible sample/assay combination modes that can set on multi-sample
nanodispenser system. Replicates can be easily done using these settings.

<b>Supplementary Table 4.
</b> Nanoliter-scale
next-generation sequencing library mediated high-throughput 16s RRNA
microbial community profiling.

All parameters tested
for SNAP-TE experiments. Two replicates of 24 nanowells on a chip were used
for each test of PCR system conditions. Data from individual next-generation
sequencing libraries from each nanowell were pooled by samples for analyses.

<b>Supplementary
Table 5. </b> Nanoliter-scale next-generation sequencing library
mediated high-throughput 16s RRNA microbial community profiling.