Intermittent treatment on exemestane acquired resistance. Intermittent treatment on exemestane acquired resistance

MCF7aro cells were cultured in hormone-stripped medium and treated with EXE continuously or intermittently (2 weeks on and 1 week off) in the presence of 1nM testosterone. At week 14, EXE-treated cells as well as MCF7aro cells were hormone-stripped for 48h were harvested and RNA was extracted.Gene expression profiles in each cell line were analyzed by the Affymetrix GeneChip Human Genome U133 Plus 2.0 arrays. Overall design: Microarray analysis of gene expression in exemestane intermittently or continuously-treated ER+ breast cancer MCF7aro cells