LFQ of biotinylated peptides from Raw264.7 cells expressing BioID-STING
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https://www.omicsdi.org/dataset/jpost/PXD009873
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The STING-null Raw264.7 cells expressing STING-BirA* were treated with DMSO or DMXAA for 15 min, and then biotin was added to the medium. After 8-h incubation, the cells were lysed and digested with trypsin. Biotinylated peptides were captured with tamavidin 2-REV magnetic beads and eluted with 2 mM biotin. The eluates were analysis by LC-MS/MS. Label-free quantification was performed using Proteome Discoverer 2.2 Software.
创建时间:
2023-04-17



