Data from: The chromatin-modifying protein HUB2 is involved in the regulation of lignin composition in xylem vessels

PIRIN2 (PRN2) was earlier reported to suppress S-type lignin accumulation
of xylem vessels of Arabidopsis (Arabidopsis thaliana). In the present
study we report yeast-two hybrid results supporting the interaction of
PRN2 with HISTONE MONOUBIQUITINATION2 (HUB2) in Arabidopsis. HUB2 has been
previously implicated in several plant developmental processes, but not in
lignification. Interaction between PRN2 and HUB2 was verified by
β-galactosidase enzymatic and co-immunoprecipitation assays. HUB2 promoted
the deposition of S-type lignin in the secondary cell walls of both stem
and hypocotyl tissues, as analysed by pyrolysis‐gas chromatography/mass
spectrometry. Chemical fingerprinting of individual xylem vessel cell
walls by Raman and Fourier Transform Infrared microspectroscopy supported
the function of HUB2 in lignin deposition. These results, together with a
genetic analyses of the hub2 prn2 double mutant, support antagonistic
function of PRN2 and HUB2 in deposition of S-type lignin. Transcriptome
analyses indicated the opposite regulation of the S-type lignin
biosynthetic FERULATE-5-HYDROXYLASE1 by PRN2 and HUB2 as the underlying
mechanism. PRN2 and HUB2 promoter activities co-localised in cells
neighbouring the xylem vessel elements, suggesting that the S-type lignin
promoting function of HUB2 is antagonized by PRN2 for the benefit of the
G-type lignin enrichment of the neighbouring xylem vessel elements.