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High-throughput RT-PCR analysis of Colon Adenocarcinoma Stem Cell Lines

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NIAID Data Ecosystem2026-03-07 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE32290
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Human early stage colon adenocarcinoma stem cell line was FACS-sorted for CCR9- and CCR9+ cell groups and differential expression was identified via Wafergen SmartChip RT-PCR gene expression profiling. CCR9- and CCR+ cells were FACS-sorted. 24 hours afterwards, cells were treated with 100ng/ml human CCL25 for 30 min. RNA was extracted from both populations using PureLink RNA Mini kit (Invitrogen) and analyzed using the SmartChip Real-Time PCR System
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2012-07-01
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