File S1 - Transposon Defense by Endo-siRNAs, piRNAs and Somatic pilRNAs in Drosophila: Contributions of Loqs-PD and R2D2

Figure S1: Backcrossing scheme of loqsko mutant in w1118 genetic background. Figure S2: Fly stock mapping of loqsko mutants. Figure S3: Backcrossing schema of r2d21 mutant in w1118 genetic background. Figure S4: Fly stock mapping of r2d2 mutants. Figure S5: Verification of β-elimination efficiency. Figure S6: Comparison of read counts in untreated and β-eliminated deep sequencing libraries (somatic RNA samples). Figure S7: Read length distribution of roo, TNFB, blood and roo transposon mapping small RNAs in r2d2 and loqs-D mutants.. Figure S8: The length distribution of 412, F-element, doc transposon mapping small RNAs in r2d2 and loqs-D mutants. Figure S9: Analysis of endo-siRNAs classified in LTRs, LINEs and IRs transposons in r2d2 and loqs-D mutants. Figure S10: Comparison of endo-siRNA abundance changes with changes in steady-state levels of transposons. Figure S11: Analysis of ping-pong signature of pilRNAs and piRNAs. Figure S12: Transcript levels of ago3, aub and piwi.Table S1: Primer sequences. (DOCX)