Translocated Legionella pneumophila small RNAs mimic eukaryotic miRNAs to dampen the host immune response [THP-1]

Extracellular vesicles (EVs) produced by bacteria, archaea and eukaryotic cells, are increasingly recognized as important mediators of intercellular communication via transfer of a wide variety of molecular cargoes. To characterize the effect of Lp-EVs on THP-1 cells, in particular the immune response, three independent RNAseq libraries were constructed from from THP-1 cells non-infected, incubated with purified wt-EVs or with purified EV from delta-rsmY Legionella bacteria. The RNA deep was purified after 3h of incubation and sequenced using an Illumina platform. Overall design: For each RNAseq experiment, the transcription of THP-1 cells incubated with extracellular vesicles deriving from wt and delta-rsmY Legionella bacteria or form the non-infected control experiment was compared. In total n=3 independent experiments were performed