Cytokines altered by Rolipram administration after spinal cord injury.

A cytokine protein array (RayBiotech Inc.) was used to identify changes in the production of 19 cytokines after SCI and/or vehicle or Rolipram administration. Fold-changes, based upon densitometry readings (normalized across arrays by positive and negative controls), indicate changes with Rolipram or vehicle treatment versus SCI controls. Symbols are as follows:(*)delivered at 1 hour post-injury;(#)delivered at 24 hours post-injury; (1) change compared to SCI control at 2 hours; (2) change compared to vehicle control at 25 hours. The changes (increase/decrease) indicated by the bolded font were subsequently confirmed using Ab-specific ELISA. Note, the following cytokines were not detected under any conditions and time points on the assay: CNTF, Fracktalkine, IFN-γ, LIX and VEGF.