RNA sequencing of small non-coding RNA from human B cell lines and their secreted exosomes

The small RNA composition in cells differs from exosomes, but underlying mechanisms have not been identified. We generated small RNA profiles by RNA-seq from a panel of human B cells and their secreted exosomes. A comprehensive bioinformatics and statistical analysis revealed non-random distributed subsets of miRNA species between B cells and exosomes. Collectively our findings suggest that the type of 3'end posttranscriptional modifications exert opposing effects that may contribute to direct ncRNA sorting into EVs.