Raw electrophysiology recording files from toads implanted with flexible mesh electronics

Background
Amphibians represent an important evolutionary transition from aquatic to terrestrial environments and they display a large variety of complex behaviors despite a relatively simple brain. However, their brain activity is not as well characterized as that of many other vertebrates, partially due to physiological traits that have made electrophysiology recordings difficult to perform in awake and moving animals.
New method
We implanted flexible mesh electronics in the cane toad (Rhinella marina) and performed extracellular recordings in the telencephalon of anesthetized toads and partially restrained, awake toads over multiple days.
Results
We recorded brain activity over five consecutive days in awake toads and over a 15 week period in a toad that was anesthetized during recordings. We were able to perform spike sorting and identified single- and multi-unit activity in all toads.
Comparison with existing methods
To our knowledge, this is the first report of a modern method to ..., This dataset includes RHD recording files from Intan RHD acquisition system and RHX software for “Chronic recording of brain activity in awake toads.” We have also included Matlab scripts that were used to process this data. 
Each file in this folder is one minute of recording data from toad that was implanted with flexible mesh electronics. The toad and the date of recording is indicated in the filename. These are representative minutes of recording that were analyzed for this paper and processed with the attached Matlab scripts., , # Raw electrophysiology recording files from toads implanted with flexible mesh electronics

[https://doi.org/10.5061/dryad.np5hqc03s](https://doi.org/10.5061/dryad.np5hqc03s)

## Description of the data and file structure

**Experiments in\"Chronic recording of brain activity in awake toads\"**

The purpose of these experiments was to overcome obstacles to establish chronic electrophysiology recordings in awake toads. In order to overcome issues related to amphibian physiology and brain movement, we implemented the use of flexible mesh electronics to record activity from the telencephalon of awake toads over five days, and from an anesthetized toad over 15 weeks. This represents a far greater duration of recording than previously achieved in amphibians, and is also one of the first successful efforts to record brain activity from awake, non-paralyzed amphibians.

### Files and variables

#### File: F02\_data.zip

**Description:** This folder holds raw RHD files that each represent one mi...,