Raw electrophysiology recording files from toads implanted with flexible mesh electronics

Background Amphibians represent an important evolutionary transition from aquatic to terrestrial environments and they display a large variety of complex behaviors despite a relatively simple brain. However, their brain activity is not as well characterized as that of many other vertebrates, partially due to physiological traits that have made electrophysiology recordings difficult to perform in awake and moving animals. New method We implanted flexible mesh electronics in the cane toad (Rhinella marina) and performed extracellular recordings in the telencephalon of anesthetized toads and partially restrained, awake toads over multiple days. Results We recorded brain activity over five consecutive days in awake toads and over a 15 week period in a toad that was anesthetized during recordings. We were able to perform spike sorting and identified single- and multi-unit activity in all toads. Comparison with existing methods To our knowledge, this is the first report of a modern method to ..., This dataset includes RHD recording files from Intan RHD acquisition system and RHX software for “Chronic recording of brain activity in awake toads.” We have also included Matlab scripts that were used to process this data.  Each file in this folder is one minute of recording data from toad that was implanted with flexible mesh electronics. The toad and the date of recording is indicated in the filename. These are representative minutes of recording that were analyzed for this paper and processed with the attached Matlab scripts., , # Raw electrophysiology recording files from toads implanted with flexible mesh electronics [https://doi.org/10.5061/dryad.np5hqc03s](https://doi.org/10.5061/dryad.np5hqc03s) ## Description of the data and file structure **Experiments in\"Chronic recording of brain activity in awake toads\"** The purpose of these experiments was to overcome obstacles to establish chronic electrophysiology recordings in awake toads. In order to overcome issues related to amphibian physiology and brain movement, we implemented the use of flexible mesh electronics to record activity from the telencephalon of awake toads over five days, and from an anesthetized toad over 15 weeks. This represents a far greater duration of recording than previously achieved in amphibians, and is also one of the first successful efforts to record brain activity from awake, non-paralyzed amphibians. ### Files and variables #### File: F02\_data.zip **Description:** This folder holds raw RHD files that each represent one mi...,研究背景 两栖动物是水生向陆生环境演化的关键过渡类群，尽管其大脑结构相对简单，却展现出丰富多样的复杂行为。然而，相较于诸多其他脊椎动物，学界对两栖动物大脑活动的表征仍不够充分，这一现状部分源于其生理特性，使得在清醒且自主活动的个体中开展电生理记录极具挑战。 新型方法 我们将柔性网状电子器件植入蔗蟾（Rhinella marina）体内，分别对麻醉状态的蔗蟾，以及轻度约束的清醒蔗蟾的端脑进行了多天数的细胞外记录。 实验结果 我们成功在清醒蔗蟾中完成了连续5天的脑活动记录，并在一只记录期间处于麻醉状态的蔗蟾中实现了长达15周的记录。我们可开展峰电位分类（spike sorting），并在所有受试蔗蟾中识别出单单位活动与多单位活动。 与现有方法的对比 据我们所知，本研究首次报道了一种适用于相关场景的现代电生理记录方法。本数据集包含来自Intan RHD采集系统与RHX软件的RHD格式记录文件，用于支撑"清醒蔗蟾脑活动的慢性记录"相关研究。我们还附带了用于处理该数据集的Matlab脚本。 本文件夹内的每个文件均对应一段时长1分钟的、来自植入柔性网状电子器件的蔗蟾的记录数据。文件名中包含了受试蔗蟾的标识信息与记录日期。这些均为经本文附带Matlab脚本处理并用于本研究分析的代表性记录片段。 # 植入柔性网状电子器件的蔗蟾原始电生理记录文件 [https://doi.org/10.5061/dryad.np5hqc03s](https://doi.org/10.5061/dryad.np5hqc03s) 数据与文件结构说明 **"清醒蔗蟾脑活动的慢性记录"实验** 本系列实验旨在攻克清醒蔗蟾慢性电生理记录的技术障碍。为解决两栖动物生理特性与脑部运动带来的记录难题，我们采用柔性网状电子器件，对清醒蔗蟾的端脑完成了为期5天的记录，并对一只麻醉状态的蔗蟾实现了长达15周的脑活动采集。本次记录时长远超此前两栖动物电生理记录的已有水平，同时也是全球范围内首次成功从清醒且未麻痹的两栖动物体内记录脑活动的尝试之一。 ### 文件与变量 #### 文件：F02_data.zip **描述：** 该文件夹包含若干段时长为1分钟的原始RHD格式记录文件……