Derivatives for structure solution of the peripheral stalk from T.thermophilus A-ATPase

Rotary ATPases couple ATP hydrolysis/synthesis with proton translocation across biological membranes and so are central components of the biological energy conversion machinery. Their peripheral stalks are essential components that counteract torque generated by rotation of the central stalk during ATP synthesis or hydrolysis.These datasets are derivatives of the peripheral stalk from T.thermophilus A-ATPase. Native crystals were soaked in Lutetium(III) acetate (2K7c_3_###.img) and Dysprosium(III) chloride (2K3#######.img).Resulting maps were used to create the pdb model 3V6I. The model was used to identify bending and twisting motions inherent within the structure that accommodate movements within the ATPase. Beamline: MX2 EPN: 3511 PDB: 3V61 Organism: Thermus thermophilus HB8 Expression System: Escherichia coli Sequence: >3V6I:B|PDBID|CHAIN|SEQUENCE GMGGLGLIKSLAEKEKQLLERLEAAKKEAEERVKRAEAEAKALLEEAEAKAKALEAQYRERERAETEALLARYRERAEAE AKAVREKAMARLDEAVALVLKEVLP旋转ATP酶（Rotary ATPases）可将ATP水解与合成过程与生物膜跨质子转运过程相偶联，是生物能量转换机制的核心组成部分。其外周柄（peripheral stalk）是关键结构组件，可抵消ATP合成或水解过程中中央柄旋转所产生的扭矩。本数据集源自嗜热栖热菌（Thermus thermophilus）A型ATP酶（A-ATPase）的外周柄。天然晶体分别经乙酸镥(III)（2K7c_3_###.img）与氯化镝(III)（2K3#######.img）浸泡处理。所得电子密度图被用于构建PDB（Protein Data Bank）模型3V6I，借助该模型可识别该结构内在的弯曲与扭转运动，此类运动可适配ATP酶内部的构象变化。 光束线：MX2 实验编号：3511 PDB编号：3V61 来源生物：嗜热栖热菌HB8（Thermus thermophilus HB8） 表达系统：大肠杆菌（Escherichia coli） 序列：>3V6I:B|PDBID|CHAIN|SEQUENCE GMGGLGLIKSLAEKEKQLLERLEAAKKEAEERVKRAEAEAKALLEEAEAKAKALEAQYRERERAETEALLARYRERAEAE AKAVREKAMARLDEAVALVLKEVLP