Regulation of glycolytic activity in multiple cell types explains the ability of FDG-PET to measure disease activity in atherosclerosis

The aim of the single cell RNASeq experiment is to explore the molecular changes in the progressing and regressing plaques of an atherosclerosis pig mode and specifically the long-term changes glycolysis pathway. To produce these glycolysis signatures, we randomized male and female pigs into two experimental groups: an actively progressing atherosclerosis group subjected to 15 months of HFHC diet (HFHC group f, n=4; m, n=2), and an LF/MTPi group fed 12 months of HFHC diet and then transitioned to low-fat diet with MTP inhibitor for three months (f, n=4; m, n=1). At the time of euthanasia, plaque from the distal 0.5 cm of the abdominal aorta was quickly processed and analyzed by scRNA-sequencing.