DUAL RNASeq ANALYSIS REVEALS AN INCREASE OF FUNGAL PATHOGENICITY FUNCTIONS AND A ROLE OF PLANT GLUTAMINE SYNTHETASE IN NITROGEN-INDUCED SUSCEPTIBILITY TO THE RICE BLAST FUNGUS

In order to understand the mechanisms of Nitrogen induced susceptibility (NIS) we’ve conducted a dual RNAseq experiment on rice infected tissues by Magnaporthe oryzae. At 0 day post inoculation and 2 days post inoculation tissues have been collected on mock inoculated and M. oryzae inoculated plants. Rice were conducted under two type of nitrogen fertilization: 0N all fertilization but nitrogen, 1N all fertilization and NH4NO3. The fertilization was applied one day before inoculation. RNAseq was conducted both on rice and fungal RNA. Overall design: shoot tissus mRNA 28-day old rice plant were generated by deep sequencing. Three samples per conditions, Four conditions: 0N Mock, 0N Inoc, 1N Mock, 1N Inoc. 0N = plant fertilized one day before inoculation without nitrogen. 1N = plant fertilized one day before inoculation with NH4NO3. Mock = Plant inoculated with control solution (gelatin 0,5%). Inoc = Plant inoculated with Magnaporthe oryzae isolate Guy11 with gelatin 0,5%.