The enzyme was preincubated for 5 min at 35°C with the various reagents before addition of the substrate. Each reagent was added at the final concentration as indicated.
casein zymographic analysis demonstrates the significant increase in MMP-12 activity (P<0.01) in COPD mice compared to control mice. This increase was significantly inhibited in mice exposed to PM2
* HCTLase activity was measured at 10 mM concentration of substrate.† Iodoacetamide inhibited rBPHL to the same extent.Inhibition of human liver HCTL activity.
Values are expressed as mean values ± standard deviation (n = 3 experiments); %, percentage;*, 8 mg/ml in the reaction medium;**, 0.4 mg/ml;***, 100 µg/ml in the reaction medium;A, B, C, D: means foll
