RNA seq-based transcriptome analysis of eccrine sweat glands from anhidrotic patients

Eccrine sweat glands contribute to the regulation of human body temperature. Acquired idiopathic generalized anhidrosis (AIGA) is extensive anhidrosis of unknown etiology, that causes the fatal heatstroke and skin dryness. Eccrine sweat glands in hidrotic and anhidrotic skin areas were excised from paraffin-embedded skin specimens from AIGA patients, and the gene expression was profiled by RNA-sequencing. We analyzed the RNA samples to clarify the difference in gene expression between hidrotic and anhidrotic skin areas from AIGA patients. Eccrine sweat glands in hidrotic and anhidrotic skin areas were excised from paraffin-embedded skin specimens from four AIGA patients, and the gene expression was profiled by RNA-sequencing. Since RNA could not be extracted from one patient due to a lack of sample material in the anhidrotic lesion, we analyzed the RNA samples derived from 3 hidrotic and 4 anhidrotic lesions.