Trx knockdown in intestinal stem cells (ISCs) of Drosophila melanogaster
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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Male flies were crossed to virgins of genotype yw;esg-Gal4,UAS-GFP;tub-Gal80ts/Tm3,Sb. Progeny was collected and aged 3-4 days before shifting for 7 days to 29 degrees to induce RNAi expression. Guts were dissected for 2 biological replicates/condition, each containing 80-100 guts, dissociated and GFP-positive cells were FACS-sorted. RNA was isolated using the PicoPure RNA-isolation kit and libraries were generated. Sequencing was done by distributing the 4 libraries over 5 lanes on the Illumina HiSeq2000 platform.
提供机构：
Roche Polska Sp. z o.o.
创建时间：
2022-02-20
