RNAseq of parental and persister cells generated human cancer cells

Persister cells (PS) were generated by diverse drug treatments of PC9 cells. Transcriptomes of these differently generated drug-persisting cells revealed common gene expression signatures related to metastasis and invasion. To test the impact of ATF4 and BCL2 effector proteins on the gene expression profiles of PS, we also investigated changes in gene expression upon BH3-mimetic treatment after gene knock-down. Enrichment analysis of differentially expressed genes (DEGs) confirmed the overall dependence on BCl-2 effector proteins and ATF4 for our previously identified pathways. 52 Samples of PC9 and HT29 cell lines treated with different treatments