Colonic mucosal injury responses

The mechanisms behind acute mucosal injury responses in humans are poorly investigated.We thus examined the acute responses to mucosal barrier injury in humans with and without UC. A standardized mucosal wound extending to the muscularis mucosa layer was inflicted in the sigmoid colon of 6 patients with quiescent UC and 6 control subjects. Macroscopic, microscopic and molecular injury responses were assessed at 24 and 48 hours by repeated high resolution imaging and collection of biopsies across the wounds which were assessed by RNA sequencing. Prospective study of 6 patients with UC in remission and 6 control subjects. Patients had a biopsy taken at 0 h, 24 h, and 48 h.The biopsies at 24 and 48 h were taken across the biopsies taken at 0 h. No replicates vere included.