Artabotrys rubriflorus (Annonaceae), a new species from Yunnan China

Artabotrys rubriflorus sp. nov. is described as a new species of Annonaceae, collected from Malipo, Yunnan Province, China.The specimens of this new species were gathered through fieldwork and were subsequently subjected to rigorous analysis using both classical taxonomical methods and molecular phylogenetic analysis. The phylogenetic reconstruction, based on four chloroplast DNA regions (matK, rbcL, trnLF, and psbAtrnH), was carried out employing maximum parsimony, maximum likelihood and Bayesian inference approaches. These analyses confirmed the systematic positioning of Artabotrys rubriflorus sp. nov. within the genus Artabotrys, while also establishing its distinctiveness from other species., Chloroplast genomic DNA was extracted from collected silica-dried leaves (Narzary et al. 2015) using the Ezup Spin Column Super Plant Genomic DNA Extraction Kit (Sangon Biotech, Shanghai). Four chloroplast DNA regions (matK, rbcL, trnL-F and psbA-trnH) were subjected to PCR amplification. Primer information is given in Table 3. The PCR reaction system (25 µl) consisted of 15 µl of 2 Taq Master Mix buffer, 10 µl of ddH2O, 3 µl of the primer mixture (1.5 µl each of the positive and reverse primer), and 2 µl of the DNA template. The parameters for PCR procedure was 95 for 3 min, followed by 40 cycles of 95 for 30 s, appropriate annealing temperature for 30 s, 72 for 1 min, and 10 min for final extension at 72 , the annealing temperatures was 54 for matK and rbcL genes, 49 for trnL-F and psbA-trnH intergenic spacers. 5 µl of the products were tested using electrophoresis in 1% agarose gel at 150 V, 110 mA for 10 to 20 min. San Prep Column DNA Gel Extraction Kit (Sangon Biotech, Shangha..., , # Data from: Artabotrys rubriflorus (Annonaceae), a new species from Yunnan China [https://doi.org/10.5061/dryad.fbg79cp62](https://doi.org/10.5061/dryad.fbg79cp62) ## Description of the data and file structure Chloroplast genomic DNA was extracted from collected silica-dried leaves (Narzary et al. 2015) using the Ezup Spin Column Super Plant Genomic DNA Extraction Kit (Sangon Biotech, Shanghai). Four chloroplast DNA regions (matK, rbcL, trnL-F and psbA-trnH) were subjected to PCR amplification. The PCR reaction system (25 µl) consisted of 15 µl of 2 Taq Master Mix buffer, 10 µl of ddH2O, 3 µl of the primer mixture (1.5 µl each of the positive and reverse primer), and 2 µl of the DNA template. The parameters for PCR procedure was 95 for 3 min, followed by 40 cycles of 95 for 30 s, appropriate annealing temperature for 30 s, 72 for 1 min, and 10 min for final extension at 72 , the annealing temperatures was 54 for matK and rbcL genes, 49 for trnL-F and psbA-trnH intergenic spacers. 5 ...,本文描述了番荔枝科（Annonaceae）一新种——红花鹰爪花（Artabotrys rubriflorus sp. nov.），该新种采集自中国云南省麻栗坡县。本新种的标本通过野外考察获取，随后采用经典分类学方法与分子系统发育分析手段对其开展了严谨的研究。 研究基于4个叶绿体DNA区域（matK、rbcL、trnLF及psbAtrnH），分别使用最大简约法、最大似然法与贝叶斯推断方法进行系统发育重建。分析结果确认了红花鹰爪花隶属于鹰爪花属（Artabotrys），同时证实了其与其他物种的区分度。 叶绿体基因组DNA从采集的硅胶干燥叶片中提取（Narzary等，2015），使用上海生工生物（Sangon Biotech）的Ezup柱式植物基因组DNA提取试剂盒完成提取。针对matK、rbcL、trnL-F及psbA-trnH这4个叶绿体DNA区域进行聚合酶链式反应（PCR）扩增，引物信息详见表3。 PCR反应体系总体积为25微升，包含15微升2×Taq Master Mix缓冲液、10微升双蒸水（ddH₂O）、3微升引物混合液（正、反向引物各1.5微升）以及2微升DNA模板。PCR扩增程序参数设置为：95℃预变性3分钟；随后进行40个循环，每个循环包括95℃变性30秒、适宜退火温度退火30秒、72℃延伸1分钟；最后于72℃终末延伸10分钟。其中matK与rbcL基因的退火温度为54℃，trnL-F及psbA-trnH基因间区的退火温度为49℃。 取5微升扩增产物，于1%琼脂糖凝胶中以150V、110mA电泳10~20分钟进行检测。胶回收使用上海生工生物的San Prep柱式DNA胶回收试剂盒。 # 数据来源：红花鹰爪花（Artabotrys rubriflorus，番荔枝科），中国云南一新种 [https://doi.org/10.5061/dryad.fbg79cp62](https://doi.org/10.5061/dryad.fbg79cp62) ## 数据与文件结构说明 叶绿体基因组DNA从采集的硅胶干燥叶片中提取（Narzary等，2015），使用上海生工生物（Sangon Biotech）的Ezup柱式植物基因组DNA提取试剂盒完成提取。针对matK、rbcL、trnL-F及psbA-trnH这4个叶绿体DNA区域进行PCR扩增。PCR反应体系总体积为25微升，包含15微升2×Taq Master Mix缓冲液、10微升ddH₂O、3微升引物混合液（正、反向引物各1.5微升）以及2微升DNA模板。PCR扩增程序参数设置为：95℃预变性3分钟；随后进行40个循环，每个循环包括95℃变性30秒、适宜退火温度退火30秒、72℃延伸1分钟；最后于72℃终末延伸10分钟。其中matK与rbcL基因的退火温度为54℃，trnL-F及psbA-trnH基因间区的退火温度为49℃。5微升……