Final analysis of data sets from two grants awarded by the Qatar National Research Fund (QNRF).

My research as summarized herein, focuses on the effects of different variables like aging, traumatic hemorrhage, ischemia and re-perfusion injures, osmotic and mechanical cellular stressors on the vascular tone control mechanisms, and on the molecular mechanisms of the associated vascular and interstitial tissue remodeling. As independent investigator during the past 7 years, I developed the State of Qatar’s first basic biomedical research that uses animals, together with the oversight committee (IACUC) for the program. This was achieved by two competitive peer reviewed grants summing $1.5 M as awarded by the Qatar National Research Fund. The work in these two grants (summarized in the two technical report documents), was centered to study the pathophysiology of the blood peritoneal barrier at the cellular and interstitial levels during acute and chronic exposures of the peritoneal cavity to sterile glucose-based peritoneal dialysis solution; separately we examined the efficacy and effectiveness in terms of short and long term outcome of direct endothelial-cytosolic energy (ATP) delivery as adjunct to resuscitation from traumatic exsanguination. The final goal from this research was to determine the molecular mechanisms of peritoneal membrane dysfunction/failure and to device methods to improve peritoneal dialysis technique survival and longevity; and to improve resuscitation outcome of progressive hemorrhagic shock.本文综述的本项研究，聚焦衰老、创伤性出血、缺血再灌注损伤、渗透压与机械性细胞应激原等多种变量对血管张力调控机制的影响，以及与之相关的血管与间质组织重塑的分子机制。过去七年间，作为独立研究者，我与该项目的实验动物管理与使用委员会（Institutional Animal Care and Use Committee, IACUC）合作，开展了卡塔尔首个使用实验动物的基础生物医学研究。该项目由卡塔尔国家研究基金会（Qatar National Research Fund）通过两项经同行评议的竞争性研究资助立项，总经费达150万美元。上述两项资助课题的相关内容已总结于两份技术报告文档中，其核心研究方向为：当腹膜腔处于急性与慢性暴露于无菌葡萄糖基腹膜透析液（peritoneal dialysis solution）的状态时，从细胞与间质层面解析血液腹膜屏障的病理生理学机制；此外，本研究还单独评估了直接向内皮细胞胞浆递送三磷酸腺苷（Adenosine Triphosphate, ATP）作为创伤失血性休克复苏辅助手段的短期与长期疗效与有效性。本研究的最终目标为：明确腹膜功能障碍/衰竭的分子机制，开发提升腹膜透析技术存续时长与使用寿命的方法，并改善进行性失血性休克的复苏效果。