Hippo signaling instructs ectopic but not normal organ growth [Drosophila YkiHyper]

In order to study how ectopic Yki drives tissue overgrowth in Drosophila imaginal discs, we overexpressed the constitutively active Yki3SA and deleted wts in clones of cells in the entire eye-antennal imaginal disc, as well as specifically in eye disc proper cells using Optix-Gal4. Using the MARCM system allowed us to compare the effects of Yki3SA overexpression in wild-type and sd mutant clones. Differential gene expression analysis of samples of eye-antennal imaginal discs with Yki hyperactivation and their corresponding controls: 1) eyFLP wts KO and eyFLP control; 2) optix>Yki3SA and optix control; 3) eyFLP MARCM Yki3SA and eyFLP MARCM ctrl as well as eyFLP MARCM Yki3SA sd- and eyFLP MARCM sd- to measure the effects of Yki hyperactivation in sd mutant clones.