The brassinosteroid receptor gene BRI1 safeguards cell-autonomous brassinosteroid signaling across tissues

Brassinosteroid signaling is essential for plant growth as exemplified by the dwarf phenotype of loss-of-function mutants in BRASSINOSTEROID INSENSITIVE 1 (BRI1), a ubiquitously expressed Arabidopsis brassinosteroid receptor gene. Complementation of brassinosteroid-blind receptor mutants by BRI1 expression with various tissue-specific promoters implied that local brassinosteroid signaling may instruct growth non-cell-autonomously. Here we performed such rescues with a panel of receptor variants and promoters, in combination with tissue-specific transgene knockouts. Our experiments demonstrate that brassinosteroid receptor expression in several tissues is necessary but not sufficient for rescue. Moreover, complementation with tissue-specific promoters requires the genuine BRI1 gene body sequence, which confers ubiquitous expression of trace receptor amounts that are sufficient to promote brassinosteroid-dependent root growth. Our data, therefore, argue for a largely cell-autonomous actio..., , , # The brassinosteroid receptor gene BRI1 safeguards cell-autonomous brassinosteroid signaling across tissues

[https://doi.org/10.5061/dryad.905qfttth](https://doi.org/10.5061/dryad.905qfttth)

Single cell RNA sequencing (scRNAseq) data obtained from root tips of different *Arabidopsis thaliana* genotypes were processed as described in the Materials and Methods to obtain gene expression quantifications. The code for processing the data is provided. 

## Description of the data and file structure

An Excel file with five different sheets: Sheet 1 contains the legend for sheets 2 to 5. Sheet 2 contains the expression analyses of the *CVP2::BRI1-CITRINE* transgenic line as compared to wild type and *bri1 brl1 brl3* triple mutants. Sheet 3 contains an excerpt of Sheet 2, focussed on *BRI1-CITRINE* and *CVP2*. Sheet 4 contains the expression analyses of the *GL2::BRI1-GFP* transgenic line as compared to wild type and *bri1 brl1 brl3* triple mutants. Sheet 5 contains an excerpt of Sheet 4, fo...