The use of micro-Computed Tomography as a minimally invasive tool for anatomical study of bivalves (Mollusca: Bivalvia). Zoological Journal of the Linnean Society, 186: 46-75.
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Micro-computed tomography or micro-CT is a minimally invasive technique capable of generating 2D images and 3D tomographic reconstructions of small-sized animals without destroying the physical specimens. The technique has been previously applied to the study of bivalves, and here we extend it to study the anatomy of eight anomalodesmatan species through the exclusive use of a tomographic approach. Freshly fixed and museum specimens of Anomalodesmata, one of the least understood clades of bivalves, were selected and scanned. Tomographic anatomical descriptions were generated for Pandora pinna (Pandoridae), Lyonsia alvarezii (Lyonsiidae), Allogramma formosa (Lyonsiellidae), Trigonulina ornata (Verticordiidae), Poromya rostrata (Poromyidae), Cetoconcha spinosula, Cetoconcha aff. smithii (Cetoconchidae) and Cuspidaria glacialis (Cuspidariidae). The main internal anatomical features often evaluated for anomalodesmatans were documented. The tomographic images allowed for a detailed description of anatomical structures, including the mantle, ctenidia, labial palps and siphons, as well as the digestive, reproductive and nervous systems. This non-destructive technique thus proved to be an efficient tool for describing the anatomy of bivalves. The potential of micro-CT in concert with the constant development of new scanners, bring fresh perspectives to the anatomical study of bivalves. Poromya spinosula is transferred to Cetoconcha and Cetoconchidae, as Cetoconcha spinosula (Thiele, 1912) comb. nov.
显微计算机断层扫描(micro-computed tomography,简称micro-CT)是一种微创成像技术,可在不破坏实体标本的前提下,获取小型动物的二维断层图像与三维断层重建结果。该技术此前已应用于双壳类动物的相关研究,本研究则仅通过断层成像方法,将其拓展用于8个异韧带亚纲(Anomalodesmata)物种的解剖结构探究。研究选取了了解程度最低的双壳类演化支之一——异韧带亚纲的新鲜固定标本与馆藏标本进行扫描,并为以下8个物种构建了断层解剖学描述:偏顶蛤(*Pandora pinna*,潘多拉蛤科Pandoridae)、阿尔瓦雷斯里昂司蛤(*Lyonsia alvarezii*,里昂司蛤科Lyonsiidae)、美丽异纹蛤(*Allogramma formosa*,里昂司细蛤科Lyonsiellidae)、装饰三棱蛤(*Trigonulina ornata*,丝盘蛤科Verticordiidae)、喙吻孔原蛤(*Poromya rostrata*,孔原蛤科Poromyidae)、刺盖丝蛤(*Cetoconcha spinosula*,盖丝蛤科Cetoconchidae)、近史密斯盖丝蛤(*Cetoconcha aff. smithii*,盖丝蛤科Cetoconchidae)以及冰川鸭嘴蛤(*Cuspidaria glacialis*,鸭嘴蛤科Cuspidariidae)。本研究记录了异韧带亚纲物种常被评估的核心内部解剖特征,断层成像图像可对多种解剖结构开展详细描述,涵盖外套膜、栉鳃、唇瓣、虹吸管,以及消化系统、生殖系统与神经系统。由此证明,该无损成像技术是描述双壳类动物解剖结构的高效工具。显微CT技术的应用潜力,结合新型扫描设备的持续发展,为双壳类的解剖学研究带来了全新视角。本研究将*Poromya spinosula*重新归入盖丝蛤属(*Cetoconcha*)及盖丝蛤科(Cetoconchidae),确立为新组合*Cetoconcha spinosula*(Thiele, 1912)comb. nov.。



