Epigenome analyses reveal histone H4K20me3 and radiation related genes in NSCLC cell

Purpose: Radiotherapy is useful for non-small cell lung cancer (NSCLC) patients who cannot be treated surgically. Modification of histone proteins also occurs during radiotherapy, and affects gene expression. In this study, we assessed the effects of radiotherapy on histone H4K20me3 in NSCLC cells. Methods: NCI-H460 NSCLC cell line were subjected to gamma irradiation. To reveal H4K20me3-related genes, we conducted chromatin immunoprecipitation(ChIP) and ChIP-sequencing. Results: We evaluated the H4K20me3-related genes through ChIP-sequencing. Overall design: We subjected NCI-H460 cells exposed to 0 or 4 Gy of radiation to ChIP using H4K20me3 antibody (Cell signaling technology,Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb #5737) and sequencing.