Transcriptomic analysis of gene expression in diosgenin producing yeast strains

To detect the true expression patterns of diosgenin (DSG) synthetic genes after pathway engineering as well as investigate the impact of the DSG accumulation on the physiological state of yeast cells, a global analysis of the transcriptomes of DSG producing strains was performed using mRNA sequencing (RNA-Seq). DSG producing strains include LP085-Vc (DSG titer of 8.0 mg/L), LP104-17 (24.6 mg/L), and LP118 (31.4mg/L) were grown in SD media (2% w/v glucose) and cell samples of the 6h and 24h fermentation time points were collected for transcriptome analyses.为探明途径工程后薯蓣皂苷元（diosgenin, DSG）合成基因的真实表达模式，并解析DSG积累对酵母细胞生理状态的影响，本研究采用mRNA测序（RNA-Seq）技术对产DSG菌株的转录组开展全局分析。所用产DSG菌株包括LP085-Vc（DSG效价8.0 mg/L）、LP104-17（24.6 mg/L）与LP118（31.4 mg/L），上述菌株均在SD培养基（2%质量体积比葡萄糖）中培养，并分别于发酵6 h、24 h时间点收集细胞样本用于转录组分析。