Novel cancer gene discovery using a forward genetic screen in RCAS-PDGFB driven gliomas

Here we describe the use of a high-throughput pipeline coupled to the commonly used tissue-specific retroviral RCAS-TVA mouse tumor model system. Utilizing next generation sequencing, we show that retroviral integration sites can be reproducibly detected in malignant stem cell lines generated from RCAS-PDGFB-driven glioma biopsies. 63 cell lines and 14 biopsies were analyzed for RCAS integrations. In addition, one control sample was sequenced.