Rhizospheric bacterial community of citrus trees expressing phloem-directed antimicrobials

This study investigates the impact of genetically modified citrus trees expressing antimicrobial proteins on soil microbiome diversity in the context of combating Huanglongbing (HLB), a devastating disease affecting the citrus industry globally. HLB, caused by Candidatus Liberibacter asiaticus (CLas), poses significant economic threats, yet effective management strategies remain elusive. Here, we focus on citrus trees genetically engineered to express the 16 kDa phloem protein (PP16) fused with antimicrobials (human lysozyme C and human beta-defensin-2) to target CLas within the plant vascular system. By employing 16S amplicon analysis, we assess the soil microbiome diversity surrounding these modified trees, aiming to understand the ecological implications of this approach. This research addresses a critical gap in understanding the potential impacts of genetically modified crops on soil microbial communities and their relevance for sustainable disease management strategies.