Gene expression analysis by bulk RNAseq of circulating hemocytes, lymph gland, and wing disc during parasitoid wasp infection.

MS Excel sheets with gene expression in circulating hemocytes (first sheet), lymph gland, and wing disc (second sheet). RNA was extracted 72 h after egg laying = time of infection = 0 h, 81 h after egg laying = 9 h postinfection/hpi and 90 h after egg laying = 18 hpi), from hemocytes, lymph glands, and wing discs of the third instar w1118 larvae. Barcoded 3′-end seq forward libraries were subjected to deep uni-directional sequencing of 75-base long reads using Illumina NextSeq. Trimmed reads in Fastq files were mapped to the BDGP Drosophila melanogaster release 6.29 genomic sequence (gene names correspond to this release) using the Mapper for RNA Seq in Geneious prime software (Biomatters). Normalized counts of reads mapped to each gene annotation were calculated as transcripts per million (TPM), expression levels were compared using the DESeq2 method in Geneious prime software. Raw data are available at The European Nucleotide Archive under Study accession number: PRJEB74490 (secondary acc: ERP159178) (https://www.ebi.ac.uk/ena/browser/view/PRJEB74490). (XLSX)