Extended Amygdala-Parabrachial Circuits Alter Threat Perception to Control Feeding

Ribosome TRAP (affinity purification) from Glutamaterigc or GABAergic neurons of the Bed Nucleus Stria Terminalis (BNST) expressing Cre, enabling expression of a floxed TRAP reporter delivered by retrofecting AAV injected into the parabrachial nucleus (PBN) of mice. Overall design: RNA-seq of dissected BNST (whole lysate, "input") and immunoprecipitate fraction ("IP") to determine genes enriched in Glutamatergic or GABAergic neurons of BNST projecting to the PBN.