Transcriptomic landscape of radiation-induced murine thyroid proliferative lesions

This study aims at the identification and characterization of early-state radiation-associated neoplastic processes by histologic and transcriptomic analyses of thyroid tissues derived from a mouse model. Comprehensive histological examination of 246 thyroids (164 exposed, 82 non-exposed) was carried out. Proliferative and normal tissues from exposed cases and normal tissue from non-exposed cases were collected by laser-capture microdissection, followed by RNAseq transcriptomic profiling using a low input 3`-library preparation protocol, differential gene expression analysis and functional association by Gene Set Enrichment Analysis. Overall design: 9 individual samples of normal thyroid tissue from non-exposed mice (nromal control tissue, NCT) 9 samples of normal thyroid tissue from exposed mice with adjacent histologic abnormalities (normal tissue, NT) 9 samples of proliferative lesions tissue (PL), paired with NT samples