Single-cell mRNA sequencing of TAC-model mouse heart tissue

We studied the cell compositon of mouse heart by single-cell sequencing on TAC-model mice. Distint subgroups of cardiac muscle, fibroblast cell and endothelial cell were detected. We drawed a cell-cell interaction network using specific expressed ligands and receptors of cells. And we also observed the change of interaction and cell transformation with progress of the disease. Extract cells from left ventricle of mouse heart and conduct single-cell sequencing