Transcriptome sequencing dataset of lung and medulla tissues from control and Asarum essential oil–treated mice

This dataset was generated from lung and medulla tissues collected at the same time point from control mice, high-dose Asarum essential oil–treated mice (600 mg/mL), and high-dose methyl eugenol–treated mice (100 mg/mL). All tissues were immediately frozen in liquid nitrogen. Total RNA was extracted using the Trizol method, and RNA quality was assessed by agarose gel electrophoresis, NanoPhotometer OD ratios, and Agilent Bioanalyzer 2100 (RNA Nano 6000 Kit). Samples with RIN ≥ 7 and RNA ≥ 800 ng were used for library construction.mRNA libraries were prepared using the Illumina NEBNext® Ultra™ RNA Library Prep Kit, including polyA+ mRNA enrichment, fragmentation, cDNA synthesis, end repair, adaptor ligation, and PCR enrichment. Library quality was evaluated by Qubit quantification, Agilent 2100 insert-size analysis, and qPCR concentration measurement (>2 nM). Qualified libraries were pooled and sequenced on the Illumina NovaSeq platform to generate 150 bp paired-end reads.Raw fastq files underwent standard quality control, and clean reads were aligned to the reference genome using HISAT2 v2.0.5. Gene-level readcounts were produced with featureCounts, and FPKM values were calculated for expression quantification. Differential expression analysis was performed with DESeq2, and normalized expression data were used for clustering.