Genome-wide maps of Ets21C and Pointed isoforms PntP1 and PntP2 binding sites in intestinal stem cells (ISCs) and transcriptome profiling of ISCs over-expressing Spitz, Ets21C, PntP1, or PntP2

Purpose: The ETS transcription factors Ets21C and Pnt are downstream effectors of EGFR signaling pathway regulating intestinal stem cell (ISC) proliferation. ISC-specific DNA binding mapping using DamID and mRNA expression profiling were performed to identify the role of Ets21C and Pnt downstream of EGFR signaling. Methods: For DamID, ISC were FACS sorted and their genomic DNA extracted, amplified, and sequenced. For RNA-Seq, ISC were FACS sorted and their total RNA extracted, amplified (only for Pnt samples), and sequenced. Conclusions: We found that Ets21C and Pnt have a role in driving both ISC proliferation and metabolic changes. Overall design: 4 biological replicates were performed for Ets21C DamID-Seq, 5 biological replicates for PntP1 and PntP2 DamID-Seq, 3 biological replicates for PntP1 RNA-Seq, 2 biological replicates for PntP2 RNA-Seq, and 3 biological replicates for Ets21C and Spitz RNA-Seq.