Fig2E_18S-gel_R1-R3_LG379-374.sgd
收藏Figshare2023-01-19 更新2026-04-08 收录
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https://figshare.com/articles/dataset/Fig2E_18S-gel_R1-R3_LG379-374_sgd/21793451/1
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<em>Xrn1 knock out A549 cells were infected with WT or PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.</em> <em>HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with or without the STOML2 99bp sequence insertion. RNA was extracted and analyzed by Northern blotting. Ethidium bromide pictures of gel showing rRNA are included.</em>
提供机构：
Gaglia, Marta
创建时间：
2023-01-19
