Transcription profiling of mouse back muscle from Six1-/-Six4-/- and control embryos in order to identify genes under the control of Six proteins at E18.5.

The aim of the experiment was to compare the transcriptome of Six1-/-Six4-/- and control embryos in order to identify genes under the control of Six proteins at E18.5. E18.5 RNAs from back muscles of three SixdKO and two control embryos were hybridized on Affymetrix mouse genome 430A2.0 arrays (Affymetrix, Strasbourg - France).本实验旨在比较Six1与Six4双基因敲除（Six1-/-Six4-/-）胚胎与对照胚胎的转录组，以鉴定胚胎发育第18.5天（E18.5）时受Six蛋白调控的基因。采集3例Six双基因敲除（SixdKO）胚胎与2例对照胚胎背部肌肉的总RNA，将其与Affymetrix小鼠基因组430A2.0芯片（Affymetrix，法国斯特拉斯堡）进行杂交实验。