Studies on the equine enteroinsular axis

Equine insulin dysregulation is a burgeoning and poorly understood problem associated with high morbidity. Based on the premise that insulin dysregulation originates in the foregut, this innovative project aimed to identify the earliest pathogenic factors of disease by investigating two key hormones: ghrelin and GLP-2; and, whether a specific genetic mutation underlies insulin dysregulation. Using innovative approaches, the project examined gastrointestinal factors influencing insulin dysregulation, which will enable the identification of at-risk animals and pinpoint novel treatment strategies. Improved disease treatment and prevention will reduce the suffering associated with painful and often lethal co-morbidities. The gastrointestinal peptides glucagon-like peptide-1 and glucagon-like peptide-2 are key components of the enteroinsular axis and have a role in metabolic diseases. They are coded by the proglucagon gene (GCG) and released from L cells in the gastrointestinal tract. Very little investigation of these peptides has been undertaken in horses, despite horses being prone to metabolic dysfunction. This dataset contains basic physiological data from in vitro studies of the physiology of these two peptides in healthy horse tissues. Initial dataset contains 2 spreadsheets: Studies on equine glucagon-like peptide 1 and Studies on equine glucagon-like peptide 2. Additional spreadsheet (GLP1 inhibitor study_raw data) uploaded 1/11/23.马胰岛素调节障碍（Equine Insulin Dysregulation）是一类快速蔓延、认知程度极低且伴随高发病率的疾病问题。本项目基于"胰岛素调节障碍起源于前肠"这一前提，旨在通过探究两种关键激素——饥饿素（ghrelin）与胰高血糖素样肽-2（GLP-2），以及是否存在特定基因突变介导该疾病，来明确其最早的致病因素。 本项目采用创新研究方法，对影响胰岛素调节障碍的胃肠道相关因素展开分析，此举将助力识别高危患病动物，并精准定位全新的治疗策略。优化疾病治疗与预防方案，将减轻由伴随的疼痛性甚至致死性合并症所带来的病痛折磨。 胃肠道肽类物质胰高血糖素样肽-1（GLP-1）与胰高血糖素样肽-2（GLP-2）是肠胰岛素轴的核心组成部分，且在代谢疾病中发挥关键作用。二者由胰高血糖素原基因（proglucagon gene, GCG）编码，并由胃肠道的L细胞释放。尽管马类易出现代谢功能异常，但目前针对此类肽类在马体内的相关研究仍极为匮乏。本数据集收录了健康马组织中这两种肽类生理特性的体外（in vitro）实验基础生理数据。 初始数据集包含2份电子表格：《马源胰高血糖素样肽-1研究》与《马源胰高血糖素样肽-2研究》。另有1份补充电子表格《GLP-1抑制剂研究原始数据》于2023年1月11日上传。