Crystal structure of the large fragment of DNA Polymerase I from Thermus aquaticus in a closed ternary complex with 5-(N-(10-hydroxydecanoyl)-aminopentenyl)-2'-deoxyuridine-triphosphate

Crystal structure of the large fragment of DNA Polymerase I from Thermus aquaticus in a closed ternary complex with 5-(N-(10-hydroxydecanoyl)-aminopentenyl)-2'-deoxyuridine-triphosphate Descriptor: 2'-deoxy-5-{(1E)-5-[(10-hydroxydecanoyl)amino]pent-1-en-1-yl}uridine 5'-(tetrahydrogen triphosphate), DNA (5'-D(*AP*AP*AP*AP*GP*GP*CP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3'), DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(DOC))-3'), ... Authors: Hottin, A, Betz, K, Marx, A. Deposit date: 2015-10-05 Release date: 2016-12-07 Last modified: 2024-01-10 Method: X-RAY DIFFRACTION (1.8 Å) Cite: Structural Basis for the KlenTaq DNA Polymerase Catalysed Incorporation of Alkene- versus Alkyne-Modified Nucleotides. Chemistry, 23, 2017

水生嗜热菌（Thermus aquaticus）来源的DNA聚合酶I（DNA Polymerase I）大片段与5-(N-(10-羟基癸酰基)-氨基戊烯基)-2'-脱氧尿苷三磷酸形成闭合三元复合物的晶体结构。描述物：2'-脱氧-5-{(1E)-5-[(10-羟基癸酰基)氨基]戊-1-烯-1-基}尿苷5'-（三氢三磷酸酯），DNA（5'-D(*AP*AP*AP*AP*GP*GP*CP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3'），DNA（5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(DOC))-3'），…… 作者：Hottin, A、Betz, K、Marx, A。寄存日期：2015-10-05；发布日期：2016-12-07；最后修改日期：2024-01-10。实验方法：X射线衍射（分辨率1.8 Å）。引用文献：《KlenTaq DNA聚合酶催化烯烃修饰与炔烃修饰核苷酸掺入的结构基础》，发表于《Chemistry》2017年第23卷。