RNA content of yeast nuclear mRNPs. RNA content of yeast nuclear mRNPs

We isolated nuclear mRNPs from yeast using an endogenous bi-molecular tagging approach on Sub2 and Hpr1. To validate the nature of these isolated particles, extracted RNA was sequenced and differential enrichment, as well as metagene coverage analysis were performed. Overall design: Two replicates each of RNA-Seq data for transcripts extracted from total lysate or from eluted particles.