Bulk RNA sequencing analysis of Ctrl/PROTAC human airway basal stem cells stimulated by TGF-ß

To evaluate how TGF-ß regulates human airway basal stem cells (BCs), we used PROTAC to inhibit TGF-ß signaling in BCs and exposed Ctrl-BCs and PROTAC-BCs to recombinant TGF-ß1 for 24 hours, followed by bulk RNA sequencing analysis. We found that multiple cell cycle related and proliferation related genes were upregulated in PROTAC-BCs compared to Ctrl-BCs, while the expression levels of cell migration related genes were reduced in the PROTAC-BCs group, and genes related to barrier formation and stem cell differentiation were also downregulated. Overall design: Ctrl-BCs and PROTAC-BCs were stimulated with 10 ng/ml recombinant TGF-ß1 in culture for 24 hours and subjected to bulk RNA sequencing. Total RNA was extracted from BCs using Trizol (15596018CN, Invitrogen), and RNA extraction was performed according to the manufacturer's instructions. Subsequently, the extracted RNA was treated with Dnase I (Invitrogen, Life Technologies, USA) to remove any contaminating DNA. Utilize the Novogene platform for CDNA library construction and sequencing.